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VPS25 antibody, Internal

Anti-VPS25 antibody, Internal used in Western Blot (WB). GTX89428
Anti-VPS25 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89428

Cat. No. GTX89428

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all VPS25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 10μg/ml
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89428-PEP).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vacuolar protein sorting 25 homolog , DERP9 , EAP20 , FAP20

Cellular Localization

Cytoplasm , Endosome membrane , nucleoplasm

Background

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-VPS25 antibody, Internal used in Western Blot (WB). GTX89428

GTX89428 WB Image

WB analysis of rat kidney lysate using GTX89428 VPS25 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-VPS25 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89428

GTX89428 IHC-P Image

IHC-P analysis of human kidney using GTX89428 VPS25 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399