GeneTex
United States (US)

VPS26 antibody [C2C3], C-term

GTX106297_WB_19011506_142.jpg
GTX106297_39953_IHC_19011506_353.jpg

Cat No. GTX106297

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS26. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ12930 antibody, HB58 antibody, Hbeta58 antibody, PEP8A antibody, VPS26 antibody, VPS26A antibody, vacuolar protein sorting-associated protein 26A antibody, vesicle protein sorting 26A antibody, vacuolar protein sorting 26 homolog A (S. pombe) antibody

Cellular Localization

Cytoplasm , Endosome membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
DATA IMAGES
GTX106297_WB_19011506_142.jpg

GTX106297 WB Image

Sample(30 µg whole cell lysate)
A:293T
B:A431(GTX27909)
C:HeLa S3(GTX14654)
D:Hep G2 (GTX27900)
10% SDS PAGE
GTX106297 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106297_39953_IHC_19011506_353.jpg

GTX106297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS26(GTX106297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169