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VPS26 antibody [C2C3], C-term

Orthogonal
Anti-VPS26 antibody [C2C3], C-term used in Western Blot (WB). GTX106297
Anti-VPS26 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106297
Anti-VPS26 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106297
Anti-VPS26 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106297

Cat. No. GTX106297

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VPS26A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS26. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FLJ12930 antibody , HB58 antibody , Hbeta58 antibody , PEP8A antibody , VPS26 antibody , VPS26A antibody , vacuolar protein sorting-associated protein 26A antibody , vesicle protein sorting 26A antibody , vacuolar protein sorting 26 homolog A (S. pombe) antibody

Cellular Localization

Cytoplasm , Endosome membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-VPS26 antibody [C2C3], C-term used in Western Blot (WB). GTX106297

GTX106297 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VPS26 antibody [C2C3], C-term (GTX106297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-VPS26 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106297

GTX106297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS26(GTX106297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS26 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106297

GTX106297 ICC/IF Image

VPS26 antibody [C2C3], C-term detects VPS26 protein at Endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS26 stained by VPS26 antibody [C2C3], C-term (GTX106297) diluted at 1:1000.

Anti-VPS26 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106297

GTX106297 ICC/IF Image

VPS26 antibody [C2C3], C-term detects VPS26 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: VPS26 stained by VPS26 antibody [C2C3], C-term (GTX106297) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Andr?s-Benito P et al. Aging (Albany NY) 2023; 15 (9) : 3295-3330 Proteostatic modulation in brain aging without associated Alzheimer's disease-and age-related neuropathological changes.
Lin G et al. Cell Metab 2018; (Epub) Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169