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VPS28 antibody, C-term

Anti-VPS28 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10133
Anti-VPS28 antibody, C-term used in Western Blot (WB). GTX10133

Cat. No. GTX10133

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all VPS28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 10μg/ml

Note :

IHC-P
Human Testis stains acrosomal head caps in round spermatids.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10133-PEP).

Product Note

This antibody is expected to recognize isoform 1 (NP_057292.1) only.

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESAYNAFNRFLHA, from the C Terminus of the protein sequence according to NP_057292.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS28 subunit of ESCRT-I

Cellular Localization

Cell membrane

Background

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-VPS28 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10133

GTX10133 IHC-P Image

IHC-P analysis of human testis using GTX10133 VPS28 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10μg/ml

Anti-VPS28 antibody, C-term used in Western Blot (WB). GTX10133

GTX10133 WB Image

WB analysis of human testis lysate using GTX10133 VPS28 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399