GeneTex
United States (US)

VPS28 antibody, C-term

GTX10133_432_IHC-P_19011506_198.jpg
GTX10133_1468_WB_19011506_659.jpg

Cat No. GTX10133

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 10μg/ml

Note :

IHC-P
Human Testis stains acrosomal head caps in round spermatids.

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10133-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform 1 (NP_057292.1) only.

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-ESAYNAFNRFLHA, from the C Terminus of the protein sequence according to NP_057292.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vps28, Escrt-I Subunit,Vps28

Background

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX10133_432_IHC-P_19011506_198.jpg

GTX10133 IHC-P Image

IHC-P analysis of human testis using GTX10133 VPS28 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10µg/ml

GTX10133_1468_WB_19011506_659.jpg

GTX10133 WB Image

WB analysis of human testis lysate using GTX10133 VPS28 antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329