Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using VPS28(GTX115937) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using VPS28(GTX115937) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX115937 diluted at 1:5000