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VPS29 antibody

Anti-VPS29 antibody used in Western Blot (WB). GTX116189
Anti-VPS29 antibody used in IHC (Paraffin sections) (IHC-P). GTX116189

Cat. No. GTX116189

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VPS29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719752

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS29 retromer complex component , DC15 , DC7 , PEP11

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Endosome membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS29 antibody used in Western Blot (WB). GTX116189

GTX116189 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX116189 diluted at 1:2000

Anti-VPS29 antibody used in IHC (Paraffin sections) (IHC-P). GTX116189

GTX116189 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using VPS29(GTX116189) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

VPS29 antibody Cat. No. GTX116189

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-VPS29 antibody used in Western Blot (WB). GTX116189
Date : Anonymous submitted on 10-Feb-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 50μg
Blocking : 5% milk/TBST, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169