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VPS34 antibody

Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Immunoprecipitation (IP). GTX129528

Cat No. GTX129528

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IP 1:100-1:500

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , unboiled hVps34-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VPS34. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphatidylinositol 3-kinase catalytic subunit type 3 , VPS34 , Vps34 , hVps34

Background

Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.

Database

Research Area

DATA IMAGES

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:1000.

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS34 antibody used in Immunoprecipitation (IP). GTX129528

GTX129528 IP Image

Immunoprecipitation of VPS34 protein from HeLa whole cell extracts using 5 μg of VPS34 antibody (GTX129528).
Western blot analysis was performed using VPS34 antibody (GTX129528).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169