Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:1000.

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of VPS34 protein from HeLa whole cell extracts using 5 μg of VPS34 antibody (GTX129528).
Western blot analysis was performed using VPS34 antibody (GTX129528).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000