APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-3μg/ml |
| Assay dependent |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX10099-PEP).
Predict Reactivity
Mouse, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Vps35, Retromer Complex Component , Mem3 , Park17 , Vps35
Background
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX10099 IHC-P Image
IHC-P analysis of human small intestine using GTX10099 VPS35 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
GTX10099 ICC/IF Image
ICC/IF analysis of PFA-fixed and saponin permeabilized SH-SY5Y using GTX10099 VPS35 antibody, C-term.
|
|
GTX10099 ICC/IF Image
ICC/IF analysis of PFA-fixed and saponin permeabilized HEK293 using GTX10099 VPS35 antibody, C-term.
|
|
GTX10099 WB Image
WB analysis of human brain (cerebellum) (A) and rat brain (B) lysates using GTX10099 VPS35 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
GTX10099 IHC-P Image
IHC-P analysis of human prostate using GTX10099 VPS35 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
REFERENCE
There are currently no references for VPS35 antibody, C-term (GTX10099). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VPS35 antibody, C-term (GTX10099). Be the first to share your experience with this product.
