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VPS35 antibody, C-term

Anti-VPS35 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10099
Anti-VPS35 antibody, C-term used in Western Blot (WB). GTX10099
Anti-VPS35 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10099
Anti-VPS35 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10099
Anti-VPS35 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10099

Cat No. GTX10099

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat, Hamster
Package
100 μg ($329)
See all VPS35 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10099-PEP).

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vps35, Retromer Complex Component,Mem3,Park17,Vps35

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-VPS35 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10099

GTX10099 ICC/IF Image

ICC/IF analysis of PFA-fixed and saponin permeabilized HEK293 using GTX10099 VPS35 antibody, C-term.
Anti-VPS35 antibody, C-term used in Western Blot (WB). GTX10099

GTX10099 WB Image

WB analysis of human brain (cerebellum) (A) and rat brain (B) lysates using GTX10099 VPS35 antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer
Anti-VPS35 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10099

GTX10099 IHC-P Image

IHC-P analysis of human prostate using GTX10099 VPS35 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml
Anti-VPS35 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10099

GTX10099 IHC-P Image

IHC-P analysis of human small intestine using GTX10099 VPS35 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml
Anti-VPS35 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10099

GTX10099 ICC/IF Image

ICC/IF analysis of PFA-fixed and saponin permeabilized SH-SY5Y using GTX10099 VPS35 antibody, C-term.
REFERENCE
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REVIEW
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Package List Price ($)
$ 329