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VPS35 antibody [C3], C-term

KO/KD
Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

Cat. No. GTX108058

Reference 6
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all VPS35 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Mouse brain , rat brain , HeLa , 293T

Predict Reactivity

Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241448

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058

GTX108058 ICC/IF Image

VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

VPS35 antibody detects VPS35 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
VPS35 antibody (GTX108058) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108058 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108058

GTX108058 IHC-P Image

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ayoubi R et al. F1000Res 2023; 12 : 452 Identification of high-performing antibodies for Vacuolar protein sorting-associated protein 35 (hVPS35) for use in Western Blot, immunoprecipitation and immunofluorescence.
Rahman AA et al. Neuroscience 2020; 441 : 33-45 Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N.
Application : WB
Reactivity : Human
Beilina A et al. Cell Rep 2020; 31 (5) : 107614 The Parkinson's Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi Network.
Application : ICC/IF
Reactivity : Human
Lenoir M et al. Nat Commun 2018; 9 (1) : 993 Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting.
Succoio M et al. J Proteomics 2015; 128 (Epub) : 18-29 Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

VPS35 antibody [C3], C-term Cat. No. GTX108058

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-VPS35 antibody [C3], C-term used in Immunoprecipitation (IP). GTX108058
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 2% starting material UB: 2% unbound fraction IP: immunoprecipitate
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. 0.5 ml aliquots at 2.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and VPS35 KO cells
Amount used : 20 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169