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GeneTex
United States (US)

VPS35 antibody [C3], C-term

KO/KD
GTX108058_39721_20150410_IFA_19011507_312.jpg
GTX108058_39721_WB_M_brain_19011507_692.jpg
GTX108058_41703_20180907_WB_shRNA_watermark_19011507_519.jpg
GTX108058_41703_WB_19011507_827.jpg
GTX108058_39721_IHC_19011507_124.jpg
GTX108058_WB_19011507_928.jpg
GTX108058_39721_WB_R_brain_19011507_639.jpg

Cat No. GTX108058

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
See all VPS35 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vps35, Retromer Complex Component,Mem3,Park17,Vps35

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108058_39721_20150410_IFA_19011507_312.jpg

GTX108058 ICC/IF Image

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 protein stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
GTX108058_39721_WB_M_brain_19011507_692.jpg

GTX108058 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108058 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108058_41703_20180907_WB_shRNA_watermark_19011507_519.jpg

GTX108058 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108058_41703_WB_19011507_827.jpg

GTX108058 WB Image

VPS35 antibody [C3], C-term detects VPS35 protein by western blot analysis.
A. 30 µg A549 whole cell extract
B. 30 µg H1299 whole cell extract
C. 30 µg HCT116 whole cell extract
7.5% SDS-PAGE
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108058_39721_IHC_19011507_124.jpg

GTX108058 IHC-P Image

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX108058_WB_19011507_928.jpg

GTX108058 WB Image

Sample(30 µg of whole cell lysate)
A:H1299
B:HeLa S3 (GTX14654)
C:Hep G2 (GTX27900)
D:MOLT4 (GTX27912)
E:Raji (GTX27908)
7.5% SDS PAGE
GTX108058 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX108058_39721_WB_R_brain_19011507_639.jpg

GTX108058 WB Image

VPS35 antibody detects VPS35 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
VPS35 antibody (GTX108058) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Lenoir M et al. Nat Commun 2018; 9 (1) : 993 Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting.
Succoio M et al. J Proteomics 2015; 128 (Epub Ahead of Print) : 18-29 Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.
Application : WB
Reactivity : Human
Miura E et al. Neurobiol Dis 2014; VPS35 dysfunction impairs lysosomal degradation of α-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169