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VPS35 antibody [C3], C-term

KO/KD
Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058
Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058
Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

Cat No. GTX108058

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all VPS35 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

Mouse brain,Rat Brain,Hela,A549,H1299,HCT116,HepG2,Molt4,Raji

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241448

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058

GTX108058 ICC/IF Image

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 protein stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-VPS35 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108058

GTX108058 ICC/IF Image

VPS35 antibody detects VPS35 protein at endosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody (GTX108058) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX108058 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108058

GTX108058 IHC-P Image

VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

VPS35 antibody detects VPS35 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
VPS35 antibody (GTX108058) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [C3], C-term used in Western Blot (WB). GTX108058

GTX108058 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Rahman AA et al. Neuroscience 2020; 441 : 33-45 Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N.
Application : WB
Reactivity : Human
Beilina A et al. Cell Rep 2020; 31 (5) : 107614 The Parkinson's Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi Network.
Application : ICC/IF
Reactivity : Human
Lenoir M et al. Nat Commun 2018; 9 (1) : 993 Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting.
Succoio M et al. J Proteomics 2015; 128 (Epub) : 18-29 Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence.
Application : WB
Reactivity : Human
Miura E et al. Neurobiol Dis 2014; VPS35 dysfunction impairs lysosomal degradation of α-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease.
Application : WB, ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169