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VPS35 antibody [HL1017]

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821
Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

Cat. No. GTX635821

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1017

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm,Endosome

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821

GTX635821 IHC-P Image

VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635821

GTX635821 ICC/IF Image

VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody (GTX635821) diluted at 1:500.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821

GTX635821 IHC-P Image

VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821

GTX635821 IHC-P Image

VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

VPS35 antibody [HL1017] Cat. No. GTX635821

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 2% starting material UB: 2% unbound fraction IP: immunoprecipitate
Figure Note : HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 μg of GTX108058 pre-coupled to beads. Samples were washed and processed for immunoblot with GTX635821. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB,IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM: 2% starting material UB: 2% unbound fraction IP: immunoprecipitate
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. 0.5 ml aliquots at 2.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and VPS35 KO cells
Amount used : 20 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Package List Price ($)
$ 399
$ 169
Custom Conjugations