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VPS35 antibody [HL1017]

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821
Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821
Anti-VPS35 antibody [HL1017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635821

Cat No. GTX635821

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1017

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888583

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm,Endosome

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in Western Blot (WB). GTX635821

GTX635821 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody [HL1017] used in IHC (Paraffin sections) (IHC-P). GTX635821

GTX635821 IHC-P Image

VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VPS35 antibody [HL1017] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635821

GTX635821 ICC/IF Image

VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody (GTX635821) diluted at 1:500.

REFERENCE

There are currently no references for VPS35 antibody [HL1017] (GTX635821). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for VPS35 antibody [HL1017] (GTX635821). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations