GeneTex
  • Country / Location Selection

United States (US)

VPS35 antibody

Anti-VPS35 antibody used in IHC (Paraffin sections) (IHC-P). GTX116260
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Anti-VPS35 antibody used in Immunoprecipitation (IP). GTX116260
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Anti-VPS35 antibody used in Western Blot (WB). GTX116260

Cat No. GTX116260

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

HCT116 , NIH-3T3 , JC

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10626418

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody used in IHC (Paraffin sections) (IHC-P). GTX116260

GTX116260 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS35(GTX116260) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody used in Immunoprecipitation (IP). GTX116260

GTX116260 IP Image

Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 μg of VPS35 antibody (GTX116260).
Western blot analysis was performed using VPS35 antibody (GTX116260).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody (GTX116260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for VPS35 antibody (GTX116260). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for VPS35 antibody (GTX116260). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169