APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HCT116 , NIH-3T3 , JC
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.64 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10626418
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
VPS35 retromer complex component , MEM3 , PARK17
Cellular Localization
Cytoplasm , Membrane; Peripheral membrane protein
Background
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX116260 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS35(GTX116260) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX116260 WB Image
Sample (30 μg of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX116260 IP Image
Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 μg of VPS35 antibody (GTX116260).
Western blot analysis was performed using VPS35 antibody (GTX116260).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX116260 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody (GTX116260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX116260 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for VPS35 antibody (GTX116260). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VPS35 antibody (GTX116260). Be the first to share your experience with this product.
