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VPS35 antibody

KO/KD
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Anti-VPS35 antibody used in IHC (Paraffin sections) (IHC-P). GTX116260

Cat. No. GTX116260

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all VPS35 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

NIH-3T3 , JC , HCT116 , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10626418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VPS35 retromer complex component , MEM3 , PARK17

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody (GTX116260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody used in Western Blot (WB). GTX116260

GTX116260 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX116260 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS35 antibody used in IHC (Paraffin sections) (IHC-P). GTX116260

GTX116260 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS35(GTX116260) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

VPS35 antibody Cat. No. GTX116260

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-VPS35 antibody used in Western Blot (WB). GTX116260
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and VPS35 KO cells
Amount used : 20 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169