GeneTex
United States (US)

VPS37C antibody [N1], N-term

GTX108053_WB_19011507_223.jpg
GTX108053_39721_IFA_19011507_710.jpg

Cat No. GTX108053

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VPS37C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vps37C, Escrt-I Subunit,Vps37C

Cellular Localization

Late endosome membrane; Peripheral membrane protein (Probable)

Background

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM]

Database

DATA IMAGES
GTX108053_WB_19011507_223.jpg

GTX108053 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX108053 diluted at 1:500

GTX108053_39721_IFA_19011507_710.jpg

GTX108053 ICC/IF Image

VPS37C antibody [N1], N-term detects VPS37C protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS37C protein stained by VPS37C antibody [N1], N-term (GTX108053) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169