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VPS4B antibody [N3C3]

Anti-VPS4B antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115249
Anti-VPS4B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115249
Anti-VPS4B antibody [N3C3] used in Western Blot (WB). GTX115249
Anti-VPS4B antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115249

Cat. No. GTX115249

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VPS4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623070

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vacuolar protein sorting 4 homolog B , MIG1 , SKD1 , SKD1B , VPS4-2

Cellular Localization

Prevacuolar compartment membrane; Peripheral membrane protein , Late endosome membrane; Peripheral membrane protein (Probable)

Background

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VPS4B antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115249

GTX115249 ICC/IF Image

VPS4B antibody [N3C3] detects VPS4B protein at nucleus and endosome by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS4B protein stained by VPS4B antibody [N3C3] (GTX115249) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-VPS4B antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115249

GTX115249 IHC-P Image

VPS4B antibody [N3C3] detects VPS4B protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
VPS4B antibody [N3C3] (GTX115249) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VPS4B antibody [N3C3] used in Western Blot (WB). GTX115249

GTX115249 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115249 diluted at 1:3000
Anti-VPS4B antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115249

GTX115249 ICC/IF Image

VPS4B antibody [N3C3] detects VPS4B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS4B protein stained by VPS4B antibody [N3C3] (GTX115249) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Salvi M et al. Arch Biochem Biophys 2014; 545C : 83-91 CK2 involvement in ESCRT-III complex phosphorylation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169