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VR1/ TRPV1 antibody

Anti-VR1/ TRPV1 antibody used in Western Blot (WB). GTX10296

Cat No. GTX10296

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Dog, Primate
Package
50 μl ($319)

APPLICATION

Application Note

ICC: Use at a dilution of 1/1000. IHC-Fr: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 100.7 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

95 kDa. ( Note )

Positive Control

VR1 transfected cells

PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic peptide: RASLDSEESESPPQENSC, corresponding to amino-terminus of Rat VR1, amino acids 4-21.

Purification

Whole antiserum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Transient Receptor Potential Cation Channel, Subfamily V, Member 1 , Trpv1_Son , Vr.5'Sv , Vr1 , Vr1L1 , Trpv1

Cellular Localization

Plasma membrane

Background

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

Database

Research Area

DATA IMAGES

Anti-VR1/ TRPV1 antibody used in Western Blot (WB). GTX10296

GTX10296 WB Image

TRPV1 in HEK293 cells was not tyrosine phosphorylated. HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1–7) or anti-TRPV1 (lanes 8–13) antibodies. Lanes 1–7 were separated from lanes 8–13 and the membranes were probed with anti-trkA antibody and anti-TRPV1 antibody, respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom)

REFERENCE

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Package List Price ($)
$ 319