WB analysis of whole cell extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), MCF7 (Lane 3), and Jurkat (Lane 4) using GTX79470 VRK1 antibody [1F6].
Dilution : 1:500

WB analysis of 25 μg of the indicated whole cell lysates using GTX79470 VRK1 antibody [1F6].
Dilution : 1:750

IHC-P analysis of human tonsil tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human liver tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human testis tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of murine cells using GTX79470 VRK1 antibody [1F6].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of U251 cells using GTX79470 VRK1 antibody [1F6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature