APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| 1:100 |
| 1:100 - 1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein GST-VRK1.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_11168498
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
VRK serine/threonine kinase 1 , PCH1 , PCH1A
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/templates/default/js/lightbox/images/loading.gif "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
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GTX79470 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature
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GTX79470 ICC/IF Image
ICC/IF analysis of U251 cells using GTX79470 VRK1 antibody [1F6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
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GTX79470 ICC/IF Image
ICC/IF analysis of murine cells using GTX79470 VRK1 antibody [1F6].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
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GTX79470 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
|
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GTX79470 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature
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GTX79470 IHC-P Image
IHC-P analysis of human testis tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200
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GTX79470 IHC-P Image
IHC-P analysis of human liver tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200
|
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GTX79470 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX79470 VRK1 antibody [1F6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes
Dilution : 1:200
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GTX79470 WB Image
WB analysis of 25 μg of the indicated whole cell lysates using GTX79470 VRK1 antibody [1F6].
Dilution : 1:750
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GTX79470 WB Image
WB analysis of whole cell extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), MCF7 (Lane 3), and Jurkat (Lane 4) using GTX79470 VRK1 antibody [1F6].
Dilution : 1:500
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REFERENCE
There are currently no references for VRK1 antibody [1F6] (GTX79470). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for VRK1 antibody [1F6] (GTX79470). Be the first to share your experience with this product.
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_879_ICC-IF_w_23061322_351.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_880_ICC-IF_w_23061322_345.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_2032_ICC-IF_w_23061322_479.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_881_ICC-IF_w_23061322_708.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_882_ICC-IF_w_23061322_411.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_1400_IHC-P_w_23061322_632.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_1399_IHC-P_w_23061322_964.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_1401_IHC-P_w_23061322_581.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_1942_WB_w_23061322_774.webp "Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470")
![Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470](/upload/website/prouct_img/small/GTX79470/GTX79470_1943_WB_w_23061322_789.webp "Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_879_ICC-IF_w_23061322_351.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_880_ICC-IF_w_23061322_345.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_2032_ICC-IF_w_23061322_479.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_881_ICC-IF_w_23061322_708.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_882_ICC-IF_w_23061322_411.webp "Anti-VRK1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_1400_IHC-P_w_23061322_632.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_1399_IHC-P_w_23061322_964.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_1401_IHC-P_w_23061322_581.webp "Anti-VRK1 antibody [1F6] used in IHC (Paraffin sections) (IHC-P). GTX79470")
![Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_1942_WB_w_23061322_774.webp "Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470")
![Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470](/upload/website/prouct_img/normal/GTX79470/GTX79470_1943_WB_w_23061322_789.webp "Anti-VRK1 antibody [1F6] used in Western Blot (WB). GTX79470")