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VRK1 antibody [5D1]

KO/KD Orthogonal
Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703
Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703
Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703
Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703
Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703
Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703
Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703

Cat. No. GTX00703

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D1

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human
Package
100 μg ($399)
See all VRK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
ICC/IF 1:100
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to N-terminus of human VRK1, MPRVKAAQAGRQSSAKRHL-C

Purification

Purified IgG
From tissue culture supernatant

Purity

90~98%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VRK serine/threonine kinase 1 , PCH1 , PCH1A

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703

GTX00703 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA

Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703

GTX00703 WB Image

KO/KD

WB analysis of HeLa cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : HeLa cells transfected Luciferase RNAi (control)
Lane 2 : HeLa cells transfected VRK1-1 RNAi
Lane 3 : HeLa cells transfected VRK1-2 RNAi
Dilution : 1:500

Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703

GTX00703 WB Image

WB analysis of various samples using GTX00703 VRK1 antibody [5D1].
Lane 1-3 : HeLa whole cell lysate
Lane 4-6 : U2OS whole cell lysate
Dilution :
Lane 1, 4 : 1:100
Lane 2, 5 : 1:500
Lane 3, 6 : 1:1000
Cell amount : 5 x10⁴ cells

Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703

GTX00703 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol

Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703

GTX00703 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol

Anti-VRK1 antibody [5D1] used in Western Blot (WB). GTX00703

GTX00703 WB Image

KO/KD

WB analysis of TIG-1 cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : TIG-1 cells transfected Luciferase RNAi (control)
Lane 2 : TIG-1 cells transfected VRK1-1 RNAi
Lane 3 : TIG-1 cells transfected VRK1-2 RNAi
Dilution : 1:500

Anti-VRK1 antibody [5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00703

GTX00703 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399