ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA

WB analysis of TIG-1 cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : TIG-1 cells transfected Luciferase RNAi (control)
Lane 2 : TIG-1 cells transfected VRK1-1 RNAi
Lane 3 : TIG-1 cells transfected VRK1-2 RNAi
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol

ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Middle and lower panel : Metaphase cells
Dilution : 1:100
Fixation : Methanol

WB analysis of various samples using GTX00703 VRK1 antibody [5D1].
Lane 1-3 : HeLa whole cell lysate
Lane 4-6 : U2OS whole cell lysate
Dilution :
Lane 1, 4 : 1:100
Lane 2, 5 : 1:500
Lane 3, 6 : 1:1000
Cell amount : 5 x10⁴ cells

WB analysis of HeLa cell lysate using GTX00703 VRK1 antibody [5D1].
Samples :
Lane 1 : HeLa cells transfected Luciferase RNAi (control)
Lane 2 : HeLa cells transfected VRK1-1 RNAi
Lane 3 : HeLa cells transfected VRK1-2 RNAi
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1].
Upper panel : Interphase cells
Lower panel : Metaphase cells
Dilution : 1:100
Fixation : 4% PFA