GeneTex
United States (US)

VRK1 antibody

Anti-VRK1 antibody used in Western Blot (WB). GTX33581
Anti-VRK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33581
Anti-VRK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33581

Cat No. GTX33581

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

45 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vrk Serine/Threonine Kinase 1,Pch1,Pch1A,Vrk1

Background

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-VRK1 antibody used in Western Blot (WB). GTX33581

GTX33581 WB Image

WB analysis of various samples using GTX33581 VRK1 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-VRK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33581

GTX33581 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33581 VRK1 antibody.
Dilution : 1:100

Anti-VRK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX33581

GTX33581 IHC-P Image

IHC-P analysis of rat lung tissue using GTX33581 VRK1 antibody.
Dilution : 1:100

Package List Price ($)
$ 329