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VRL1 antibody [C2C3], C-term

Orthogonal
Anti-VRL1 antibody [C2C3], C-term used in Western Blot (WB). GTX101868
Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868
Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868
Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868
Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868

Cat. No. GTX101868

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VRL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human VRL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily V member 2 , VRL , VRL-1 , VRL1

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cytoplasm , Melanosome

Background

This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VRL1 antibody [C2C3], C-term used in Western Blot (WB). GTX101868

GTX101868 WB Image

Orthogonal

Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [C2C3], C-term (GTX101868) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868

GTX101868 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung, using VRL1(GTX101868) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868

GTX101868 IHC-P Image

The data was published in the journal PLoS One in 2017.PMID: 28081185

Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868

GTX101868 IHC-P Image

The data was published in the journal PLoS One in 2017.PMID: 28081185

Anti-VRL1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101868

GTX101868 IHC-P Image

The data was published in the journal PLoS One in 2017.PMID: 28081185

REFERENCE

Application Reference

Sakakibara A et al. PLoS One 2017; 12 (1) : e0169723 Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169