GeneTex
United States (US)

VTA1 antibody

GTX107286_WB_19011506_455.jpg
GTX107286_IHC_19011506_840.jpg
GTX107286_39995_IFA_19011506_709.jpg

Cat No. GTX107286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VTA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vesicle Trafficking 1,C6Orf55,Drg-1,Drg1,Hspc228,Lip5,My012,Sbp1,Vta1

Cellular Localization

Cytoplasm , Endosome membrane; Peripheral membrane protein

Background

C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM]

Database

DATA IMAGES
GTX107286_WB_19011506_455.jpg

GTX107286 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
C:MOLT4 (GTX27912)
12% SDS PAGE
GTX107286 diluted at 1:1000

GTX107286_IHC_19011506_840.jpg

GTX107286 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1(GTX107286) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107286_39995_IFA_19011506_709.jpg

GTX107286 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1(GTX107286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169