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VTA1 antibody

Anti-VTA1 antibody used in Western Blot (WB). GTX107286
Anti-VTA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107286
Anti-VTA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107286

Cat. No. GTX107286

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VTA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , Molt-4

Predict Reactivity

Mouse, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VTA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952562

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle trafficking 1 , C6orf55 , DRG-1 , DRG1 , HSPC228 , LIP5 , My012 , SBP1

Cellular Localization

Cytoplasm , Endosome membrane; Peripheral membrane protein

Background

C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-VTA1 antibody used in Western Blot (WB). GTX107286

GTX107286 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
C:MOLT4 (GTX27912)
12% SDS PAGE
GTX107286 diluted at 1:1000

Anti-VTA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107286

GTX107286 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1(GTX107286) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VTA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107286

GTX107286 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1(GTX107286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE

Application Reference

Skowyra ML et al. Science 2018; 360 (6384) Triggered recruitment of ESCRT machinery promotes endolysosomal repair.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169