GeneTex
United States (US)

VWF antibody [3E2D10]

GTX35165_20170712_IHC-P_1_18121410_993.jpg
GTX35165_20170712_IHC-P_18121410_714.jpg

Cat No. GTX35165

Host Mouse
Clonality Monoclonal
Clone Name 3E2D10
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
IP 0.5-1 μg/500ug protein Lysate

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

309 kDa. ( Note )

Specificity/Sensitivity

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposiā€™s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human vWF fragment spanning aa 845-949

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Von Willebrand Factor,F8Vwf,Vwd,Vwf

Background

The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX35165_20170712_IHC-P_1_18121410_993.jpg

GTX35165 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Monoclonal Antibody (3E2D10)

GTX35165_20170712_IHC-P_18121410_714.jpg

GTX35165 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with vWF Monoclonal Antibody (3E2D10)

Package List Price ($)
$ 329