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VWF antibody [GT1230]

Recombinant antibody Orthogonal
Anti-VWF antibody [GT1230] used in Western Blot (WB). GTX02827
Anti-VWF antibody [GT1230] used in Western Blot (WB). GTX02827
Anti-VWF antibody [GT1230] used in IHC (Paraffin sections) (IHC-P). GTX02827

Cat. No. GTX02827

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1230

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all VWF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

309 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human VWF

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

von Willebrand factor , F8VWF , VWD

Cellular Localization

Secreted

Background

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-VWF antibody [GT1230] used in Western Blot (WB). GTX02827

GTX02827 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VWF antibody [GT1230] (GTX02827) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-VWF antibody [GT1230] used in Western Blot (WB). GTX02827

GTX02827 WB Image

WB analysis of mouse liver tissue lysates using GTX02827 VWF antibody [GT1230].
Dilution : 1:1000
Loading : 25μg

Anti-VWF antibody [GT1230] used in IHC (Paraffin sections) (IHC-P). GTX02827

GTX02827 IHC-P Image

IHC-P analysis of human esophageal tissue section using GTX02827 VWF antibody [GT1230].
Dilution : 1:100

REFERENCE

Application Reference

Huang LT et al. Pediatr Neonatol 2021; Roxadustat attenuates hyperoxia-induced lung injury by upregulating proangiogenic factors in newborn mice.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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Package List Price ($)
$ 419