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VWF antibody [HL2058]

Recombinant antibody Orthogonal
Anti-VWF antibody [HL2058] used in IHC (Paraffin sections) (IHC-P). GTX637962
Anti-VWF antibody [HL2058] used in Western Blot (WB). GTX637962

Cat. No. GTX637962

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2058

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Cat
Package
100 μl ($399),
25 μl ($169)
See all VWF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

309 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human VWF

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

von Willebrand factor , F8VWF , VWD

Cellular Localization

Secreted

Background

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-VWF antibody [HL2058] used in IHC (Paraffin sections) (IHC-P). GTX637962

GTX637962 IHC-P Image

VWF antibody [HL2058] detects secreted VWF protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
VWF stained by VWF antibody [HL2058] (GTX637962) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VWF antibody [HL2058] used in Western Blot (WB). GTX637962

GTX637962 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VWF antibody [HL2058] (GTX637962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations