GeneTex
United States (US)

Valyl tRNA synthetase antibody [N1N2], N-term

GTX115015_40247_IHC_18121410_446.jpg
GTX115015_WB_18121410_526.jpg
GTX115015_40247_IFA_18121410_941.jpg

Cat No. GTX115015

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Valyl tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Valyl-Trna Synthetase,G7A,Ndmsca,Vars1,Vars2,Vars

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq]

Database

DATA IMAGES
GTX115015_40247_IHC_18121410_446.jpg

GTX115015 IHC-P Image

Valyl tRNA synthetase antibody [N1N2], N-term detects Valyl tRNA synthetase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Valyl tRNA synthetase antibody [N1N2], N-term (GTX115015) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115015_WB_18121410_526.jpg

GTX115015 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
5% SDS PAGE
GTX115015 diluted at 1:10000

GTX115015_40247_IFA_18121410_941.jpg

GTX115015 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Valyl tRNA synthetase(GTX115015) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169