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Vamp2 antibody

Anti-Vamp2 antibody used in Western Blot (WB). GTX132130
Anti-Vamp2 antibody used in IHC (Whole mount) (IHC-Wm). GTX132130
Anti-Vamp2 antibody used in Immunohistochemistry (IHC). GTX132130

Cat. No. GTX132130

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Vamp2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

HEK transfected with vamp2-eGFP

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Vamp2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886574

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle-associated membrane protein 2 , wu:fj61c06 , zgc:73302

Database

Research Area

DATA IMAGES

Anti-Vamp2 antibody used in Western Blot (WB). GTX132130

GTX132130 WB Image

Vamp2 antibody detects Vamp2 protein by Western blot analysis.
A. Wholle cell lysate from untransfected HEK
B. Whole cell lysate from HEK transfected with vamp2-eGFP.
Vamp2 antibody (GTX132130) dilution: 1:1000.

Anti-Vamp2 antibody used in IHC (Whole mount) (IHC-Wm). GTX132130

GTX132130 IHC-Wm Image

The data was published in the journal Nat Commun in 2018.PMID: 29643351

Anti-Vamp2 antibody used in Immunohistochemistry (IHC). GTX132130

GTX132130 IHC Image

The data was published in the journal Front Cell Dev Biol in 2018. PMID: 30258843

REFERENCE

Application Reference

Denans N et al. Nat Commun 2022; 13 (1) : 5356 An anti-inflammatory activation sequence governs macrophage transcriptional dynamics during tissue injury in zebrafish.
Kindt KS et al. Front Cell Dev Biol 2018; 6 : 114 Transmission Disrupted: Modeling Auditory Synaptopathy in Zebrafish.
Zhang Q et al. Nat Commun 2018; 9 (1) : 1388 Synaptically silent sensory hair cells in zebrafish are recruited after damage.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

Vamp2 antibody Cat. No. GTX132130

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Vamp2 antibody used in Western Blot (WB). GTX132130
Date : Anonymous submitted on 26-Jan-2015
Score :
Application Tested : WB
Sample Species : Zfsh
Sample : HEK cells transfected with zebrafish VAMP2-eGFP
Amount used : 30mm dish with HEK cells, 13ul loaded
Blocking : 5% nondairy dry milk in 0.1% PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1:Wholle cell lysate from untransfected HEK. Lane 2:Whole cell lysate from HEK transfected with zebrafish VAMP2-eGFP.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169