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Vamp2 antibody

Anti-Vamp2 antibody used in Western Blot (WB). GTX132130

Cat No. GTX132130

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Wm
Reactivity Zebrafish
Package
200 μl ($389),
50 μl ($199)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
Not tested in other applications.

Positive Control

HEK transfected with zebrafish vamp2-eGFP
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Vamp2

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vesicle-associated membrane protein 2,wu:fj61c06,zgc:73302

Database

Research Area

DATA IMAGES
Anti-Vamp2 antibody used in Western Blot (WB). GTX132130

GTX132130 WB Image

Vamp2 antibody detects Vamp2 protein by Western blot analysis.
A. Wholle cell lysate from untransfected HEK
B. Whole cell lysate from HEK transfected with vamp2-eGFP.
Vamp2 antibody (GTX132130) dilution: 1:1000.

REFERENCE
REVIEW

Vamp2 antibody Cat No. GTX132130

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Jan-2015
Score :
Application Tested : WB
Sample Species : Zfsh
Sample : HEK cells transfected with zebrafish VAMP2-eGFP
Amount used : 30mm dish with HEK cells, 13ul loaded
Blocking : 5% nondairy dry milk in 0.1% PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1:Wholle cell lysate from untransfected HEK. Lane 2:Whole cell lysate from HEK transfected with zebrafish VAMP2-eGFP.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 389
$ 199