Identification of IE62 protein in VZV-infected cells by western blotting using anti-VZV IE62 antibody (clone 62B). Lane 1; VZV strain pOka infected MRC-5 cell lysate Lane 2; MRC-5 cell lysate ( uninfected negative control ) The anti-VZV IE62 antibody was used at 1/5,000 dilution

Immunofluorescence staining of VZV IE62 protein in VZV-infected MRC-5 cells by using anti-VZV IE62 antibody (clone 62B). Anti-VZV IE62 antibody was used at 1/100 dilution. As second antibody, Alexa Fluor 488 donkey anti-mouse IgG [H+L] was used at 1/200 dilution. Nuclei were stained with Hoechst 33342.