Vasa antibody [HL2485] detects Vasa protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Vasa stained by Vasa antibody [HL2485] (GTX638835) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Zebrafish embryo extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.