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Vasa antibody [HL2485]

Recombinant antibody
Anti-Vasa antibody [HL2485] used in IHC (Whole mount) (IHC-Wm). GTX638835
Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835
Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835
Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835

Cat. No. GTX638835

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2485

Isotype

IgG

Application

WB, IHC-Wm

Reactivity

Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Observed MW

77 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant fragment of zebrafish Vasa

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 , vas , vasa , vlg , wu:fi24g05 , zgc:109812 , zgc:158535

Database

Research Area

DATA IMAGES

Anti-Vasa antibody [HL2485] used in IHC (Whole mount) (IHC-Wm). GTX638835

GTX638835 IHC-Wm Image

Vasa antibody [HL2485] detects Vasa protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Vasa stained by Vasa antibody [HL2485] (GTX638835) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835

GTX638835 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835

GTX638835 WB Image

Zebrafish embryo extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody [HL2485] used in Western Blot (WB). GTX638835

GTX638835 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody [HL2485] (GTX638835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for Vasa antibody [HL2485] (GTX638835). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations