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Vasa antibody

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

Cat. No. GTX128306

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IHC

Reactivity

Zebrafish, Japanese Medaka, Fish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Positive Control

Zebrafish 10 hpf

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Vasa. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2847856

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 , vas , vasa , vlg , wu:fi24g05 , zgc:109812 , zgc:158535

Database

Research Area

DATA IMAGES

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

vasa antibody detects vasa protein at primordial germ cell on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
vasa antibody (GTX128306) dilution: 1:200.

Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

Vasa antibody detects Vasa protein by western blot analysis.
A. 30 μg Zebrafish 10 hpf lysate/extract
7.5% SDS-PAGE
Vasa antibody (GTX128306) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

Vasa antibody detects Vasa protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Vasa antibody (GTX128306) dilution: 1:100.

REFERENCE

REVIEW

Vasa antibody Cat. No. GTX128306

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2018.PMID: 30399145
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28211534
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169