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Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]

Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in Lateral Flow (Lateral Flow). GTX00863
Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in Western Blot (WB). GTX00863
Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in ELISA (ELISA). GTX00863

Cat No. GTX00863

Host

Mouse

Clonality

Monoclonal

Clone Name

vero-01

Isotype

IgG1

Application

WB, ELISA, Lateral Flow

Reactivity

Campylobacter jejuni, E. coli, Staphylococcus aureus, Salmonella Enteritidis
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ELISA Assay dependent
Lateral Flow Assay dependent
Not tested in other applications.

Specificity/Sensitivity

It reacts with subunit A of VT1, VT2 and Stx.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Culture supernatant of E. coli 0111 ( EHEC strain)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Database

Research Area

DATA IMAGES

Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in Lateral Flow (Lateral Flow). GTX00863

GTX00863 Lateral Flow Image

Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.

Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in Western Blot (WB). GTX00863

GTX00863 WB Image

WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2.
Lane 1 : Culture medium of E. coli O157:H7
Lane 2 : Purified VT1
Lane 3 : Purified VT2
Lane 4 : VT non-producing E. coli

Anti-Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] used in ELISA (ELISA). GTX00863

GTX00863 ELISA Image

ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

REFERENCE

There are currently no references for Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] (GTX00863). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01] (GTX00863). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 369