Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]

Cat. No. GTX00863

Host	Mouse
Clonality	Monoclonal
Clone Name	vero-01
Isotype	IgG1
Application	WB, ELISA, Lateral Flow
Reactivity	Campylobacter jejuni, E. coli, Staphylococcus aureus, Salmonella Enteritidis
Package	100 μg ($399)

See all E. coli Shiga toxin products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ELISA	Assay dependent
Lateral Flow	Assay dependent

Not tested in other applications.

Product Note

It reacts with subunit A of VT1, VT2 and Stx.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Culture supernatant of E. coli 0111 ( EHEC strain)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Database

UniProt: P08026
UniProt: P09385
UniProt: Q9FBI2

Research Area

DATA IMAGES

GTX00863 Lateral Flow Image

Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.

GTX00863 WB Image

WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2.
Lane 1 : Culture medium of E. coli O157:H7
Lane 2 : Purified VT1
Lane 3 : Purified VT2
Lane 4 : VT non-producing E. coli

GTX00863 ELISA Image

ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf

Package

List Price ($)

100 μg

$ 399

Qty

Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]

Cat. No. GTX00863

Host

Clonality

Clone Name

Isotype

Application

Reactivity