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Versican antibody

Anti-Versican antibody used in IHC (Paraffin sections) (IHC-P). GTX03733
Anti-Versican antibody used in Western Blot (WB). GTX03733

Cat. No. GTX03733

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all Versican products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr Assay dependent

Note :

IHC-P
Antigen retireval by heat
Not tested in other applications.

Calculated MW

373 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix

Background

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-Versican antibody used in IHC (Paraffin sections) (IHC-P). GTX03733

GTX03733 IHC-P Image

IHC-P analysis of human glioma tissue using GTX03733 Versican antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution)
Dilution : 1μg/ml

Anti-Versican antibody used in Western Blot (WB). GTX03733

GTX03733 WB Image

WB analysis of various sample lysates using GTX03733 Versican antibody.
Lane 1: Rat Brain(50 μg)
Lane 2: HEPA cell (40 μg)
Dilution : 0.5 μg/mL

REFERENCE

Application Reference

Q Yang et al. Adv Sci (Weinh) 2024; : e2400480 Extracellular Matrix Remodeling Alleviates Memory Deficits in Alzheimer’s Disease by Enhancing the Astrocytic Autophagy-Lysosome Pathway
Application : WB, IHC-Fr
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Package List Price ($)
$ 429