GeneTex
United States (US)

Vesicular Acetylcholine Transporter antibody

GTX133251_42648_20180604_IHC-Fr_M_18121410_532.jpg
GTX133251_42648_20171127_IHC-P_M_18121410_499.jpg
GTX133251_42648_20170803_WB_M_eye_18121410_840.jpg

Cat No. GTX133251

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Mouse Vesicular Acetylcholine Transporter. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Solute Carrier Family 18 (Vesicular Monoamine), Member 3,Vacht,Vat,Slc18A3

Database

Research Area

DATA IMAGES
GTX133251_42648_20180604_IHC-Fr_M_18121410_532.jpg

GTX133251 IHC-Fr Image

Vesicular Acetylcholine Transporter antibody detects Vesicular Acetylcholine Transporter protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Vesicular Acetylcholine Transporter stained by Vesicular Acetylcholine Transporter antibody (GTX133251) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX133251_42648_20171127_IHC-P_M_18121410_499.jpg

GTX133251 IHC-P Image

Vesicular Acetylcholine Transporter antibody detects Vesicular Acetylcholine Transporter protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: Vesicular Acetylcholine Transporter protein stained by Vesicular Acetylcholine Transporter antibody (GTX133251) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133251_42648_20170803_WB_M_eye_18121410_840.jpg

GTX133251 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vesicular Acetylcholine Transporter antibody (GTX133251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26226617

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169