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Vezatin antibody

Anti-Vezatin antibody used in Western Blot (WB). GTX116240
Anti-Vezatin antibody used in Western Blot (WB). GTX116240
Anti-Vezatin antibody used in Western Blot (WB). GTX116240
Anti-Vezatin antibody used in IHC (Paraffin sections) (IHC-P). GTX116240

Cat. No. GTX116240

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Vezatin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

HeLa , mouse brain , rat brain , mouse eye

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Vezatin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10622377

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vezatin, adherens junctions transmembrane protein , VEZATIN

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell junction , adherens junction , Nucleus , Acrosome

Background

Plays a pivotal role in the establishment of adherens junctions and their maintenance in adult life. In case of Listeria infection, promotes bacterial internalization by participating to myosin VIIa recruitment to the entry site.

Database

Research Area

DATA IMAGES

Anti-Vezatin antibody used in Western Blot (WB). GTX116240

GTX116240 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vezatin antibody (GTX116240) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vezatin antibody used in Western Blot (WB). GTX116240

GTX116240 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vezatin antibody (GTX116240) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vezatin antibody used in Western Blot (WB). GTX116240

GTX116240 WB Image

Sample (20 ug of whole cell lysate )
A: Hela
7.5% SDS PAGE
GTX116240 diluted at 1:1000

Anti-Vezatin antibody used in IHC (Paraffin sections) (IHC-P). GTX116240

GTX116240 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Vezatin(GTX116240) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169