Detection of TRH toxin in the culure medium of V. parahaemolyticus (TRH+) by western blotting with anti-TRH antibody. 1. Ten-times condensed sample were analyzed by SDS-PAGE and stained with CBB. 2. The same sample was analyzed by western blotting. The antibody at 1/2,000 dilution was used. TRH toxin band is indicated by an arrow

Anti-TRH antibody cross-reacts with TDH toxin in western blot. 1. Partially purified TDH toxin 2. Condensed culture medim of V. haemolyticus. Antibody was used at 1/2,000 dilution for WB.

Immunoprecipitation of TRH toxin from Vibrio parahaemolyticus (TRH+) culture medium with anti-TRH antibody. 1. TRH was precipitated with anti-TRH antibody from the same sample as in Fig.1, analysed by SDS-PAGE and stained with CBB. 2. TRH was precipitated with anti-TRH antibody from the same sample and TRH was detected by WB with anti-TRH antibody. Arrow indicates TRH band. H and L indicate heavy and light chain of IgG, respectively