APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human Villin protein, and it does not cross react with human Advillin, Gelsolin, and Villin like protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragmemt of human Villin
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
villin 1 , D2S1471 , VIL
Cellular Localization
Cytoplasm, cytoskeleton,Cell projection, lamellipodium,Cell projection, filopodium
Background
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/templates/default/js/lightbox/images/loading.gif "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
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GTX637555 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:4000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637555 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637555 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637555 IHC-P Image
Villin antibody [HL1830] detects Villin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Villin stained by Villin antibody [HL1830] (GTX637555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Villin antibody [HL1830] (GTX637555). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Villin antibody [HL1830] (GTX637555). Be the first to share your experience with this product.
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_44970_20230707_WB_competitor_watermark_23071300_644.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_T-44823_20230210_WB_multiple_B_23021401_696.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_44970_20230317_WB_TPM_watermark_23032022_712.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_T-44823_20230714_IHC-P_M_23080901_576.webp "Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_44970_20230707_WB_competitor_watermark_23071300_644.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_T-44823_20230210_WB_multiple_B_23021401_696.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_44970_20230317_WB_TPM_watermark_23032022_712.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_T-44823_20230714_IHC-P_M_23080901_576.webp "Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555")