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Villin antibody [MSVA-459R] HistoMAX™

Recombinant antibody HistoMAX
Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400
Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400
Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400

Cat. No. GTX04400

Host

Rabbit

Clonality

Monoclonal

Clone Name

MSVA-459R

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
500 μl ($629),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

Negative Control

Colon: Villin staining should be absent in all non-epithelial cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa600-700) of human Villin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

villin 1 , D2S1471 , VIL

Cellular Localization

Cytoplasm, cytoskeleton , Cell projection, lamellipodium , Cell projection, filopodium

Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400

GTX04400 IHC-P Image

IHC-P analysis of human renal cortex tissue using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
A strong villin positivity is seen in proximal tubuli of the kidney but not in distal tubuli or glomeruli.

Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400

GTX04400 IHC-P Image

IHC-P analysis of human mucinous ovarian cancer (MOC) tissue using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
Mucinous ovarian cancer showing strong villin immunostaining.

Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400

GTX04400 IHC-P Image

IHC-P analysis of human mucosa from descending colon tissue using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
Colon epithelial cells show a strong predominantly membranous but also cytoplasmic villin immunostaining.

REFERENCE

There are currently no references for Villin antibody [MSVA-459R] HistoMAX™ (GTX04400). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Villin antibody [MSVA-459R] HistoMAX™ (GTX04400). Be the first to share your experience with this product.
Package List Price ($)
$ 629
$ 299