PRODUCT
Summary
This antibody was validated on 76 different Normal and cancerTissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.
Negative Control
Colon: Villin staining should be absent in all non-epithelial cells.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa600-700) of human Villin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
villin 1 , D2S1471 , VIL
Cellular Localization
Cytoplasm, cytoskeleton , Cell projection, lamellipodium , Cell projection, filopodium
Background
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/templates/default/js/lightbox/images/loading.gif "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
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GTX04400 IHC-P Image
IHC-P analysis of human kidney cortex tissue section using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
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GTX04400 IHC-P Image
IHC-P analysis of human mucinous ovarian cancer tissue section using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
Mucinous ovarian cancer showing strong villin immunostaining.
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GTX04400 IHC-P Image
IHC-P analysis of human colon descendens mucosa tissue section using GTX04400 Villin antibody [MSVA-459R] HistoMAX™.
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REFERENCE
There are currently no references for Villin antibody [MSVA-459R] HistoMAX™ (GTX04400). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Villin antibody [MSVA-459R] HistoMAX™ (GTX04400). Be the first to share your experience with this product.
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/small/GTX04400/GTX04400_20230728_IHC-P_126_23072722_399.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/small/GTX04400/GTX04400_20230728_IHC-P_256_23072723_760.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/small/GTX04400/GTX04400_20230728_IHC-P_386_23072723_928.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/normal/GTX04400/GTX04400_20230728_IHC-P_126_23072722_399.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/normal/GTX04400/GTX04400_20230728_IHC-P_256_23072723_760.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")
![Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400](/upload/website/prouct_img/normal/GTX04400/GTX04400_20230728_IHC-P_386_23072723_928.webp "Anti-Villin antibody [MSVA-459R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04400")