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Villin antibody

Comparison Orthogonal
Anti-Villin antibody used in Western Blot (WB). GTX109940
Anti-Villin antibody used in Western Blot (WB). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in Western Blot (WB). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in Western Blot (WB). GTX109940
Anti-Villin antibody used in Western Blot (WB). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940
Anti-Villin antibody used in IHC (Frozen sections) (IHC-Fr). GTX109940

Cat. No. GTX109940

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Villin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

rat kidney , mouse kidney , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

villin 1 , D2S1471 , VIL

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Villin antibody used in Western Blot (WB). GTX109940

GTX109940 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody (GTX109940) diluted at 1:5000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Villin antibody used in Western Blot (WB). GTX109940

GTX109940 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX109940) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Villin (GTX109940) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects Villin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Villin stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in Western Blot (WB). GTX109940

GTX109940 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX109940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects VIL1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
VIL1 stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects VIL1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
VIL1 stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects VIL1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
VIL1 stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in Western Blot (WB). GTX109940

GTX109940 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX109940) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Villin antibody used in Western Blot (WB). GTX109940

GTX109940 WB Image

Villin antibody detects VIL1 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
7.5% SDS-PAGE
Villin antibody (GTX109940) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects Villin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Villin stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109940

GTX109940 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Villin(GTX109940) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects Villin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Villin stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects Villin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Villin stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX109940

GTX109940 IHC-P Image

Villin antibody detects Villin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Villin stained by Villin antibody (GTX109940) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in IHC (Frozen sections) (IHC-Fr). GTX109940

GTX109940 IHC-Fr Image

Orthogonal

The data was published in the journal Nat Commun in 2019. PMID: 31676794

REFERENCE

Application Reference

Reed M et al. Nat Commun 2019; 10 (1) : 5004 Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo.
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169