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Villin antibody

Comparison
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX110034
Anti-Villin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110034
Anti-Villin antibody used in Western Blot (WB). GTX110034
Anti-Villin antibody used in Western Blot (WB). GTX110034
Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX110034
Anti-Villin antibody used in Western Blot (WB). GTX110034
Anti-Villin antibody used in Western Blot (WB). GTX110034

Cat. No. GTX110034

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Villin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

H1299 , mouse kidney , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Villin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952553

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

villin 1 , D2S1471 , VIL

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX110034

GTX110034 IHC-P Image

Villin antibody detects Villin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Villin stained by Villin antibody (GTX110034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110034

GTX110034 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using Villin(GTX110034) antibody at 1:500 dilution.

Anti-Villin antibody used in Western Blot (WB). GTX110034

GTX110034 WB Image

Comparison

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody (GTX110034) diluted at 1:1000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Villin antibody used in Western Blot (WB). GTX110034

GTX110034 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX110034 diluted at 1:10000

Anti-Villin antibody used in IHC (Paraffin sections) (IHC-P). GTX110034

GTX110034 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Villin (GTX110034) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Villin antibody used in Western Blot (WB). GTX110034

GTX110034 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX110034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Villin antibody used in Western Blot (WB). GTX110034

GTX110034 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody (GTX110034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cizkova K et al. Int J Mol Sci 2023; 24 (9) Fibrates Affect Levels of Phosphorylated p38 in Intestinal Cells in a Differentiation-Dependent Manner.
Cizkova K et al. Biomedicines 2021; 9 (9) When Activator and Inhibitor of PPARα Do the Same: Consequence for Differentiation of Human Intestinal Cells.
Application : ELISA
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169