Villin antibody detects Villin protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Villin protein stained by Villin antibody (GTX133791) diluted at 1:500.
Blue: Hoechst 33342 staining.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody (GTX133791) diluted at 1:1000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody (GTX133791) diluted at 1:1000 and competitor's antibody (sc-7672) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.