Vimentin antibody [GT1180]

Name: Vimentin antibody [GT1180]
Brand: GeneTex
SKU: GTX00942
Price: 399.00 USD

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942

Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942

Anti-Vimentin antibody [GT1180] used in Immunoprecipitation (IP). GTX00942

Cat. No. GTX00942

Reference 1

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1180
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all Vimentin products

View product citations for antibody GTX00942 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IP	1:50 - 1:200

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Vimentin.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin , CTRCT30 , HEL113 , VIM , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23185433

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 WB Image

WB analysis of various samples using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Loading : 25μg

GTX00942 IHC-P Image

IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 IP Image

IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

GTX00942 WB Image

WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Laoding : 25μg

GTX00942 IHC-P Image

IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

GTX00942 IHC-P Image

IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

REFERENCE

Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.

Application : WB

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REVIEW

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Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.

Application : WB

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

Vimentin antibody [GT1180]

Cat. No. GTX00942

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX00942 WB Image

GTX00942 WB Image

GTX00942 ICC/IF Image

GTX00942 WB Image

GTX00942 IHC-P Image

GTX00942 ICC/IF Image

GTX00942 ICC/IF Image

GTX00942 IP Image

GTX00942 WB Image

GTX00942 IHC-P Image

GTX00942 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.