Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Laoding : 25μg

IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

WB analysis of various samples using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Loading : 25μg

ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100