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Vimentin antibody [GT1180]

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942
Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942
Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942
Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942
Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942
Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942
Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942
Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942
Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942
Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942
Anti-Vimentin antibody [GT1180] used in Immunoprecipitation (IP). GTX00942

Cat No. GTX00942

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1180

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.3), 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Vimentin.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin , CTRCT30 , HEL113 , VIM , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23185433

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

GTX00942 WB Image

WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Laoding : 25μg

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

GTX00942 WB Image

WB analysis of various samples using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Loading : 25μg

Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942

GTX00942 IHC-P Image

IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942

GTX00942 IHC-P Image

IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942

GTX00942 IHC-P Image

IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-Vimentin antibody [GT1180] used in Immunoprecipitation (IP). GTX00942

GTX00942 IP Image

IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

There are currently no references for Vimentin antibody [GT1180] (GTX00942). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Vimentin antibody [GT1180] (GTX00942). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf