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Vimentin antibody [GT812]

Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in IHC (Paraffin sections) (IHC-P). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744
Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

Cat. No. GTX629744

Host

Mouse

Clonality

Monoclonal

Clone Name

GT812

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA

Reactivity

Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:75000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629744, Detection : GTX112661 / GTX100619

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , C2C12

Product Note

IP/MS validation was supported by references (PMID:30377427)

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2814972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Blue: DAPI.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:3000.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

Anti-Vimentin antibody [GT812] used in IHC (Paraffin sections) (IHC-P). GTX629744

GTX629744 IHC-P Image

Vimentin antibody [GT812] detects Vimentin protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody [GT812] (GTX629744) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:75000.

Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Vimentin antibody [GT812] detects Vimentin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody [GT812] (GTX629744) dilution: 1:1000

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX629744) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

Vimentin antibody [GT812] Cat. No. GTX629744

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Sep-2016
Score :
Application Tested : FACS
Sample Species : Hu
Sample : HT29 cells
Blocking : 1% skim milk, 4°C, 1Hr
Fixation : 70% EtOH, -20°C, 1Hr
Permeabilization /Antigen retrieval : 70% EtOH, -20°C, 1hrHr
Primary Antibody : 1:200, 4°C, 1Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations