Vimentin antibody [GT812]

Name: Vimentin antibody [GT812]
Brand: GeneTex
SKU: GTX629744
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

Anti-Vimentin antibody [GT812] used in IHC (Paraffin sections) (IHC-P). GTX629744

Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744

Cat. No. GTX629744

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	GT812
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA
Reactivity	Human, Mouse, Rat, Cat
Package	100 μl ($399), 25 μl ($169)

See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:75000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629744, Detection : GTX112661 / GTX100619

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , C2C12

Product Note

IP/MS validation was supported by references (PMID:30377427)

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2814972

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Blue: DAPI.

GTX629744 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744 IHC-P Image

Vimentin antibody [GT812] detects Vimentin protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody [GT812] (GTX629744) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:75000.

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX629744) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744 WB Image

Vimentin antibody [GT812] detects Vimentin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody [GT812] (GTX629744) dilution: 1:1000

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:3000.

REFERENCE

Application Reference

Wanandi SI et al. J Pak Med Assoc 2021; 71(Suppl 2) (2) : S18-S24 Cancer-associated fibroblast (CAF) secretomes-induced epithelial-mesenchymal transition on HT-29 colorectal carcinoma cells associated with hepatocyte growth factor (HGF) signalling.

Application : ICC/IF

Reactivity : Human

Liu L et al. Mol Cancer Ther 2019; (Epub) Transgelin 2 promotes paclitaxel resistance, migration and invasion of breast cancer by directly interacting with PTEN and activating PI3K/Akt/GSK-3帣 pathway.

Lee SW et al. Biosens Bioelectron 2019; 142 : 111509 Dual-function nanostructured platform for isolation of nasopharyngeal carcinoma circulating tumor cells and EBV DNA detection.

Application : ICC/IF

Reactivity : Human

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.

Application : IP

Reactivity : Human

Liu MC et al. PLoS One 2017; 12 (5) : e0178152 Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model.

Application : ICC/IF

Reactivity : Human

Show More +

Submit a Reference

REVIEW