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Vimentin antibody [GT812]

GTX629744_41421_20150703_IHC-Fr_M_18121411_136.jpg
GTX629744_41421_20160921_IHC-Fr_18121411_801.jpg
GTX629744_41421_20160616_WB_shRNA_watermark_18121411_657.jpg
GTX629744_41421_WB_18121411_607.jpg
GTX629744_41421_20161013_WB_M_18121411_776.jpg
GTX629744_41421_20160505_WB_18121411_648.jpg
GTX629744_41421_20160706_IHC-P_R_18121411_100.jpg
GTX629744_41421_20160602_WB_R_18121411_408.jpg

Cat No. GTX629744

Host Mouse
Clonality Monoclonal
Clone Name GT812
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:75000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat-2

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377427)

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vimentin,Vim

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX629744_41421_20150703_IHC-Fr_M_18121411_136.jpg

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Blue: DAPI.

GTX629744_41421_20160921_IHC-Fr_18121411_801.jpg

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX629744_41421_20160616_WB_shRNA_watermark_18121411_657.jpg

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744_41421_WB_18121411_607.jpg

GTX629744 WB Image

Vimentin antibody [GT812] detects Vimentin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody [GT812] (GTX629744) dilution: 1:1000

GTX629744_41421_20161013_WB_M_18121411_776.jpg

GTX629744 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:3000.

GTX629744_41421_20160505_WB_18121411_648.jpg

GTX629744 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744_41421_20160706_IHC-P_R_18121411_100.jpg

GTX629744 IHC-P Image

Vimentin antibody [GT812] detects Vimentin protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody [GT812] (GTX629744) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629744_41421_20160602_WB_R_18121411_408.jpg

GTX629744 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:75000.

REFERENCE
REVIEW

Vimentin antibody [GT812] Cat No. GTX629744

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Sep-2016
Score :
Application Tested : FACS
Sample Species : Hu
Sample : HT29 cells
Blocking : 1% skim milk, 4°C, 1Hr
Fixation : 70% EtOH, -20°C, 1Hr
Permeabilization /Antigen retrieval : 70% EtOH, -20°C, 1hrHr
Primary Antibody : 1:200, 4°C, 1Hr
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169