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Vimentin antibody [HL1506]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980
Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980
Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980
Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980
Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980
Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980
Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980
Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980
Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980

Cat No. GTX636980

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1506

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

GTX636980 WB Image

KO/KD

Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

GTX636980 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

GTX636980 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980

GTX636980 ICC/IF Image

Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

GTX636980 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

GTX636980 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations