Vimentin antibody [HL1506]

Name: Vimentin antibody [HL1506]
Brand: GeneTex
SKU: GTX636980
Price: 399.00 USD

Anti-Vimentin antibody [HL1506] used in Western Blot (WB). GTX636980

Anti-Vimentin antibody [HL1506] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636980

Anti-Vimentin antibody [HL1506] used in IHC (Paraffin sections) (IHC-P). GTX636980

Cat. No. GTX636980

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1506
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Cat
Package	100 μl ($399), 25 μl ($169)

See all Vimentin products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX636980 WB Image

Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636980 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636980 ICC/IF Image

Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636980 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat lymph node.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636980 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636980 WB Image

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636980 IHC-P Image

Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

Vimentin antibody [HL1506]

Cat. No. GTX636980

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX636980 WB Image

GTX636980 WB Image

GTX636980 ICC/IF Image

GTX636980 WB Image

GTX636980 IHC-P Image

GTX636980 IHC-P Image

GTX636980 IHC-P Image

GTX636980 IHC-P Image

GTX636980 WB Image

GTX636980 WB Image

GTX636980 IHC-P Image

GTX636980 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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