APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1,000 |
| 1:20-1:200 |
| 1:20 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Human rhabdosarcoma cell line JR1.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
vimentin
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton
Background
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/templates/default/js/lightbox/images/loading.gif "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
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GTX80814 ICC/IF Image
ICC/IF analysis of HEK293 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
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GTX80814 ICC/IF Image
ICC/IF analysis of HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours using GTX80814 Vimentin antibody [J144].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
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GTX80814 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000
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GTX80814 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000
|
|
|
GTX80814 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
|
|
|
GTX80814 ICC/IF Image
ICC/IF analysis of C6 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
|
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GTX80814 WB Image
WB analysis of 20 ug of indicated whole cell lysates using GTX80814 Vimentin antibody [J144].
Dilution : 1:500
|
REFERENCE
There are currently no references for Vimentin antibody [J144] (GTX80814). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Vimentin antibody [J144] (GTX80814). Be the first to share your experience with this product.
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_920_ICC-IF_19011519_710.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_918_ICC-IF_19011519_984.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_1419_IHC-P_19011519_325.jpg "Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814")
![Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_1420_IHC-P_19011519_144.jpg "Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_921_ICC-IF_19011519_718.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_919_ICC-IF_19011519_962.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814](/upload/website/prouct_img/small/GTX80814/GTX80814_1960_WB_19011519_939.jpg "Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_920_ICC-IF_19011519_710.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_918_ICC-IF_19011519_984.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_1419_IHC-P_19011519_325.jpg "Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814")
![Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_1420_IHC-P_19011519_144.jpg "Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_921_ICC-IF_19011519_718.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_919_ICC-IF_19011519_962.jpg "Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814")
![Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814](/upload/website/prouct_img/normal/GTX80814/GTX80814_1960_WB_19011519_939.jpg "Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814")