Vimentin antibody [J144]

Cat. No. GTX80814

Host	Mouse
Clonality	Monoclonal
Clone Name	J144
Isotype	IgM
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Dog
Package	200 μl ($339)

See all Vimentin products

Datasheet
Document

View product citations for antibody GTX80814 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:20-1:200
IHC-P	1:20
IHC	Assay dependent

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human rhabdosarcoma cell line JR1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX80814 ICC/IF Image

ICC/IF analysis of C6 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX80814 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX80814 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

GTX80814 ICC/IF Image

ICC/IF analysis of HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours using GTX80814 Vimentin antibody [J144].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

GTX80814 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

GTX80814 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX80814 WB Image

WB analysis of 20 ug of indicated whole cell lysates using GTX80814 Vimentin antibody [J144].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

200 μl

$ 339

Qty

Vimentin antibody [J144]

Cat. No. GTX80814

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX80814 ICC/IF Image

GTX80814 ICC/IF Image

GTX80814 IHC-P Image

GTX80814 ICC/IF Image

GTX80814 IHC-P Image

GTX80814 ICC/IF Image

GTX80814 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.