WB analysis of 20 ug of indicated whole cell lysates using GTX80814 Vimentin antibody [J144].
Dilution : 1:500

ICC/IF analysis of HeLa cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

ICC/IF analysis of HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours using GTX80814 Vimentin antibody [J144].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

IHC-P analysis of human tonsil tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

ICC/IF analysis of HEK293 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of C6 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC