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Vimentin antibody [J144]

Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814
Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814
Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814
Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814
Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814
Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814
Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814

Cat No. GTX80814

Host

Mouse

Clonality

Monoclonal

Clone Name

J144

Isotype

IgM

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Rat, Dog
Package
200 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:20-1:200
IHC-P 1:20
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human rhabdosarcoma cell line JR1.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814

GTX80814 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814

GTX80814 ICC/IF Image

ICC/IF analysis of HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours using GTX80814 Vimentin antibody [J144].
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814

GTX80814 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

Anti-Vimentin antibody [J144] used in IHC (Paraffin sections) (IHC-P). GTX80814

GTX80814 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX80814 Vimentin antibody [J144].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814

GTX80814 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Vimentin antibody [J144] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80814

GTX80814 ICC/IF Image

ICC/IF analysis of C6 cells using GTX80814 Vimentin antibody [J144]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-Vimentin antibody [J144] used in Western Blot (WB). GTX80814

GTX80814 WB Image

WB analysis of 20 ug of indicated whole cell lysates using GTX80814 Vimentin antibody [J144].
Dilution : 1:500

REFERENCE

There are currently no references for Vimentin antibody [J144] (GTX80814). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Vimentin antibody [J144] (GTX80814). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339