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Vimentin antibody [LN-6]

Anti-Vimentin antibody [LN-6] used in IHC (Paraffin sections) (IHC-P). GTX35162

Cat No. GTX35162

Host Mouse
Clonality Monoclonal
Clone Name LN-6
Isotype IgM
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFPā€™s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.ĀVimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.ĀAb-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Human thymic nuclear extract

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vimentin,Vim

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES
Anti-Vimentin antibody [LN-6] used in IHC (Paraffin sections) (IHC-P). GTX35162

GTX35162 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).

Package List Price ($)
$ 329