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Vimentin antibody [LN-6]

Anti-Vimentin antibody [LN-6] used in IHC (Paraffin sections) (IHC-P). GTX35162

Cat. No. GTX35162

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LN-6

Isotype

 IgM

Application

 IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Pig
Package
100 μg ($529)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 0.1-0.2μg/ml for 30 min at RT
FACS Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human thymic nuclear extract

Purification

Purified IgM

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTRCT30 , HEL113 , VIM , vimentin , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [LN-6] used in IHC (Paraffin sections) (IHC-P). GTX35162

GTX35162 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX35162 Vimentin antibody [LN-6].

REFERENCE

Clone Reference

A Calle et al. Stem Cell Res Ther 2018; 9 (1):178 Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation
Application : FACS
D Dai et al. AJNR Am J Neuroradiol 2005; 26 (8):1932 - 6 Modified Histologic Technique for Processing Metallic Coil-Bearing Tissue
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529