PRODUCT
Summary
This antibody was validated on 76 different Normal and cancerTissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
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Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.
Negative Control
Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A recombinant fragment (around aa 2-466) of human Vimentin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
vimentin
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton
Background
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/templates/default/js/lightbox/images/loading.gif "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
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GTX04418 IHC-P Image
IHC-P analysis of human duodenum mucosa tissue section using GTX04418 Vimentin antibody [MSVA-458R] HistoMAX™.
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GTX04418 IHC-P Image
IHC-P analysis of human squamous cell carcinoma tissue section using GTX04418 Vimentin antibody [MSVA-458R] HistoMAX™.
Strong vimentin positivity of the tumor stroma in a squamous cell carcinoma.
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GTX04418 IHC-P Image
IHC-P analysis of human endometrium tissue section using GTX04418 Vimentin antibody [MSVA-458R] HistoMAX™.
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REFERENCE
There are currently no references for Vimentin antibody [MSVA-458R] HistoMAX™ (GTX04418). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Vimentin antibody [MSVA-458R] HistoMAX™ (GTX04418). Be the first to share your experience with this product.
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/small/GTX04418/GTX04418_20230728_IHC-P_127_23072722_546.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/small/GTX04418/GTX04418_20230728_IHC-P_257_23072723_263.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/small/GTX04418/GTX04418_20230728_IHC-P_387_23072723_621.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/normal/GTX04418/GTX04418_20230728_IHC-P_127_23072722_546.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/normal/GTX04418/GTX04418_20230728_IHC-P_257_23072723_263.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")
![Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418](/upload/website/prouct_img/normal/GTX04418/GTX04418_20230728_IHC-P_387_23072723_621.webp "Anti-Vimentin antibody [MSVA-458R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04418")