Vimentin antibody [SPM576]

Cat. No. GTX35161

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM576
Isotype	IgG1
Application	WB, IHC-P, FACS
Reactivity	Human, Goat, Bovine, Cat, Dog, Chicken, Pig
Package	100 μg ($529)

See all Vimentin products

Datasheet
Document

View product citations for antibody GTX35161 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human vimentin recombinant protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTRCT30 , HEL113 , VIM , vimentin , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Gene ID: 7431 VIM
UniProt: P08670 VIM

Research Area

DATA IMAGES

GTX35161 WB Image

WB analysis of A375 cell lysate using GTX35161 Vimentin antibody [SPM576].

GTX35161 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX35161 Vimentin antibody [SPM576].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty