*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
HeLa , rat basophilic , chicken postnatal erythrocytes , skin fibroblast , 3T3
Preservative: 15mM Sodium Azide. Constituents: PBS. pH 7.4
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Full length native protein (purified).
Purified by precipitation methods and size-exclusion chromatography
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
FLJ36605, P08670, 193060, Vimentin, 7431, VIM
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
GTX40346 IP Image
Immunoprecipitation of vimentin from HeLa cell lysate by Vimentin antibody [VI-10] and its detection by vimentin antibody [VI-01]. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)
GTX40346 ICC/IF Image
Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin antibody, vimentin (green) with Vimentin antibody [VI-10]. Nuclei are stained with DAPI (blue).
GTX40346 ICC/IF Image
Immunofluorescence staining of RBL rat basophilic cell line with Dyomics 547 conjugated Vimentin antibody [VI-10]. Nuclei are stained with DAPI (blue).