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Vimentin antibody [VI-10]

Anti-Vimentin antibody [VI-10] used in Immunoprecipitation (IP). GTX40346
Anti-Vimentin antibody [VI-10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40346

Cat No. GTX40346

Host

Mouse

Clonality

Monoclonal

Clone Name

VI-10

Isotype

IgM

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Chicken, Pig
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Purified by precipitation and chromatography

Conjugation

Unconjugated

RRID

AB_425224

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CTRCT30 , HEL113 , VIM , vimentin , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [VI-10] used in Immunoprecipitation (IP). GTX40346

GTX40346 IP Image

IP analysis of HeLa cell lysate using GTX40346 Vimentin antibody [VI-10] . The signal wass detected by GTX27752 Vimentin antibody [VI-01] in WB assay.
Lr : Lysate (reducing conditions)
Lnr : Lysate (non-reducing conditions)
IPr : Immunoprecipitate (reducing conditions)
IPnr : Immunoprecipitate (non-reducing conditions)

Anti-Vimentin antibody [VI-10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40346

GTX40346 ICC/IF Image

ICC/IF analysis of chicken postnatal erythrocytes using GTX40346 Vimentin antibody [VI-10].
Green : Primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339