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United States (US)

Vimentin antibody [VI-10]

Vimentin-antibody-VI-10-GTX40346-IP-1_18121411_841.jpg
Vimentin-antibody-VI-10-GTX40346-ICC_IF-1_18121411_538.jpg
Vimentin-antibody-VI-10-GTX40346-ICC_IF-2_18121411_945.jpg

Cat No. GTX40346

Host Mouse
Clonality Monoclonal
Clone Name VI-10
Isotype IgM
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Chicken, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa , rat basophilic , chicken postnatal erythrocytes , skin fibroblast , 3T3
PROPERTIES

Form

Liquid

Buffer

Preservative: 15mM Sodium Azide. Constituents: PBS. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Full length native protein (purified).

Purification

Purified by precipitation methods and size-exclusion chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ36605, P08670, 193060, Vimentin, 7431, VIM

Cellular Localization

Cytoplasmic

Background

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
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Database

Research Area

DATA IMAGES
Vimentin-antibody-VI-10-GTX40346-IP-1_18121411_841.jpg

GTX40346 IP Image

Immunoprecipitation of vimentin from HeLa cell lysate by Vimentin antibody [VI-10] and its detection by vimentin antibody [VI-01]. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

Vimentin-antibody-VI-10-GTX40346-ICC_IF-1_18121411_538.jpg

GTX40346 ICC/IF Image

Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin antibody, vimentin (green) with Vimentin antibody [VI-10]. Nuclei are stained with DAPI (blue).

Vimentin-antibody-VI-10-GTX40346-ICC_IF-2_18121411_945.jpg

GTX40346 ICC/IF Image

Immunofluorescence staining of RBL rat basophilic cell line with Dyomics 547 conjugated Vimentin antibody [VI-10]. Nuclei are stained with DAPI (blue).

Reference