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United States (US)

Vimentin antibody [VM1170]

GTX35163_20170712_IHC-P_2_18121410_369.jpg
GTX35163_20170712_IHC-P_1_18121410_382.jpg
GTX35163_20170712_IHC-P_18121410_659.jpg
GTX35163_20170712_IHC-P_3_18121410_877.jpg

Cat No. GTX35163

Host Mouse
Clonality Monoclonal
Clone Name VM1170
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.25-0.5ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.ĀAnti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human vimentin protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Vimentin,Vim

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES
GTX35163_20170712_IHC-P_2_18121410_369.jpg

GTX35163 IHC-P Image

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).

GTX35163_20170712_IHC-P_1_18121410_382.jpg

GTX35163 IHC-P Image

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).

GTX35163_20170712_IHC-P_18121410_659.jpg

GTX35163 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).

GTX35163_20170712_IHC-P_3_18121410_877.jpg

GTX35163 IHC-P Image

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).

Package List Price ($)
$ 329