APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
We do not recommend use of this product for Mouse,Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human vimentin protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CTRCT30 , HEL113 , VIM , vimentin , Vimentin
Cellular Localization
Cytoplasm
Background
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/templates/default/js/lightbox/images/loading.gif "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
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GTX35163 IHC-P Image
IHC-P analysis of human rhabdomyosarcoma tissue using GTX35163 Vimentin antibody [VM1170].
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GTX35163 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX35163 Vimentin antibody [VM1170].
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GTX35163 IHC-P Image
IHC-P analysis of human Ewing's sarcoma tissue using GTX35163 Vimentin antibody [VM1170].
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GTX35163 IHC-P Image
IHC-P analysis of human angiosarcoma tissue using GTX35163 Vimentin antibody [VM1170].
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GTX35163 WB Image
WB analysis of HepG2 cell lysate using GTX35163 Vimentin antibody [VM1170].
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GTX35163 IHC-P Image
IHC-P analysis of human leiomyosarcoma tissue using GTX35163 Vimentin antibody [VM1170].
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GTX35163 IHC-P Image
IHC-P analysis of human uterus tissue using GTX35163 Vimentin antibody [VM1170].
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REFERENCE
There are currently no references for Vimentin antibody [VM1170] (GTX35163). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Vimentin antibody [VM1170] (GTX35163). Be the first to share your experience with this product.
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_1098_w_23060819_634.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_790_w_23060819_631.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_567_w_23060819_228.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_281_w_23060819_945.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in Western Blot (WB). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_WB_238_w_23060819_525.webp "Anti-Vimentin antibody [VM1170] used in Western Blot (WB). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_665_w_23060819_400.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/small/GTX35163/GTX35163_20200115_IHC-P_1442_w_23060819_747.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_1098_w_23060819_634.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_790_w_23060819_631.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_567_w_23060819_228.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_281_w_23060819_945.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in Western Blot (WB). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_WB_238_w_23060819_525.webp "Anti-Vimentin antibody [VM1170] used in Western Blot (WB). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_665_w_23060819_400.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")
![Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163](/upload/website/prouct_img/normal/GTX35163/GTX35163_20200115_IHC-P_1442_w_23060819_747.webp "Anti-Vimentin antibody [VM1170] used in IHC (Paraffin sections) (IHC-P). GTX35163")