*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
We do not recommend use of this product for Mouse,Rat samples.
10mM PBS, 0.05% BSA
0.05% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant full-length human vimentin protein
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CTRCT30 , HEL113 , VIM , vimentin , Vimentin
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
GTX17723 WB Image
WB analysis of A375 cell lysate using GTX17723 Vimentin antibody [VM452].
GTX17723 FACS Image
FACS analysis of Jurkat cells using GTX17723 Vimentin antibody [VM452].
Black : Blank
Grey : Isotype Control
Green : Primary antibody
GTX17723 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
GTX17723 WB Image
WB analysis of Raji cell lysate using GTX17723 Vimentin antibody [VM452].
GTX17723 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX17723 Vimentin antibody [VM452].