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Vimentin antibody

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04497
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX04497
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04497
Anti-Vimentin antibody used in Western Blot (WB). GTX04497
Anti-Vimentin antibody used in Western Blot (WB). GTX04497

Cat. No. GTX04497

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human VIM(Accession P08670), corresponding to amino acid residues R424-E466.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04497

GTX04497 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX04497 Vimentin antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX04497

GTX04497 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX04497 Vimentin antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:200

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04497

GTX04497 ICC/IF Image

ICC/IF analysis of PFA fixed A549 cells using GTX04497 Vimentin antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-Vimentin antibody used in Western Blot (WB). GTX04497

GTX04497 WB Image

WB analysis of mouse lung tissue lysates using GTX04497 Vimentin antibody. The lane on the left was treated with blocking peptide.

Anti-Vimentin antibody used in Western Blot (WB). GTX04497

GTX04497 WB Image

WB analysis of various samples using GTX04497 Vimentin antibody.
Lane 1 : Rat muscle tissue lysates with blocking peptide treatment
Lane 2 : Rat muscle tissue lysates
Lane 3 : serum starvated B16f10 whole cell lysates

REFERENCE

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REVIEW

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Package List Price ($)
$ 399