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Vimentin antibody

Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Immunoprecipitation (IP). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in ELISA (ELISA). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619
Anti-Vimentin antibody used in ELISA (ELISA). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100619
Anti-Vimentin antibody used in Western Blot (WB). GTX100619

Cat No. GTX100619

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, Sandwich ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA Assay dependent
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629743/GTX629744, Detection: GTX100619

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Observed MW

56 kDa. The observed M.W. is based on the following publication. PMID: 23185433 and 24926698

Positive Control

293T , HeLa , MDA-MB-231 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NIH-3T3 , A549*

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: Vimentin protein stained by Vimentin antibody (GTX100619) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody (GTX100619) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Vimentin antibody detects Vimentin protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody (GTX100619) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:2000.

Anti-Vimentin antibody used in Immunoprecipitation (IP). GTX100619

GTX100619 IP Image

Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody (GTX100619).
Western blot analysis was performed using Vimentin antibody (GTX100619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in ELISA (ELISA). GTX100619

GTX100619 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Tsai CH and colleagues in the journal Sci Rep in 2017.PMID: 28195146

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 . PMID: 31174567

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

The IHC-P analysis of Vimentin antibody was published by Otti GR and colleagues in the journal PLoS One in 2014.PMID: 25397403

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Chiu JH and colleagues in the journal J Transl Med in 2017.PMID: 28472954

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Pon JR and colleagues in the journal Nat Commun in 2015.PMID: 26245647

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

The WB analysis of Vimentin antibody was published by Chiu JH and colleagues in the journal J Transl Med in 2017.PMID: 28472954

Anti-Vimentin antibody used in ELISA (ELISA). GTX100619

GTX100619 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX100619

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100619

GTX100619 ICC/IF Image

Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Vimentin antibody used in Western Blot (WB). GTX100619

GTX100619 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Vimentin antibody Cat No. GTX100619

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date : Anonymous submitted on 16-Nov-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Dental pulp stem cells
Blocking : 5% NGS in 0.1% Triton-X in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.1% Triton-X in PBS
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:50000, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf