GeneTex
United States (US)

Vimentin antibody

KO/KD
GTX100619_42095_20170522_IHC-P_M_19011506_284.jpg
GTX100619_42816_20170926_IHC-P_R_19011506_727.jpg
GTX100619_41570_20151223_IHC-P_19011506_734.jpg
GTX100619_41570_WB_Sensitivity_19011506_887.jpg
GTX100619_42158_20161110_WB_shRNA_watermark_19011506_571.jpg
GTX100619_42816_20170926_IHC-P_M_19011506_523.jpg
GTX100619_43131_20180307_ICC_IF_19011506_146.jpg
GTX100619_42669_20170926_IHC-P_R_19011506_836.jpg
GTX100619_42851_20170525_WB_19011506_869.jpg
GTX100619_42158_20160630_WB_M_19011506_554.jpg
GTX100619_40667_IHC_19011506_234.jpg
GTX100619_40667_IHC_R_19011506_549.jpg
GTX100619_41570_20150209_IP_19011506_537.jpg

Cat No. GTX100619

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Observed MW

56 kDa. The observed M.W. is based on the following publication. PMID: 23185433 and 24926698

Positive Control

293T , HeLa , MDA-MB-231 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NIH-3T3 , A549*

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.14mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ36605 antibody, VIM antibody, vimentin antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100619_42095_20170522_IHC-P_M_19011506_284.jpg

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: Vimentin protein stained by Vimentin antibody (GTX100619) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100619_42816_20170926_IHC-P_R_19011506_727.jpg

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100619_41570_20151223_IHC-P_19011506_734.jpg

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100619_41570_WB_Sensitivity_19011506_887.jpg

GTX100619 WB Image

Vimentin antibody detects Vimentin protein by western blot analysis.
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody (GTX100619) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100619_42158_20161110_WB_shRNA_watermark_19011506_571.jpg

GTX100619 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000.

GTX100619_42816_20170926_IHC-P_M_19011506_523.jpg

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100619_43131_20180307_ICC_IF_19011506_146.jpg

GTX100619 ICC/IF Image

Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100619_42669_20170926_IHC-P_R_19011506_836.jpg

GTX100619 IHC-P Image

Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100619_42851_20170525_WB_19011506_869.jpg

GTX100619 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100619_42158_20160630_WB_M_19011506_554.jpg

GTX100619 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:2000.

GTX100619_40667_IHC_19011506_234.jpg

GTX100619 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin(GTX100619) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100619_40667_IHC_R_19011506_549.jpg

GTX100619 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Vimentin(GTX100619) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100619_41570_20150209_IP_19011506_537.jpg

GTX100619 IP Image

Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody (GTX100619).
Western blot analysis was performed using Vimentin antibody (GTX100619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
REVIEW

Vimentin antibody Cat No. GTX100619

Rating ( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Date : Anonymous submitted on 16-Nov-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Dental pulp stem cells
Blocking : 5% NGS in 0.1% Triton-X in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.1% Triton-X in PBS
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 05-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 20μg
Blocking : 5% skim milk in TBST, 24°C, 1Hr
Primary Antibody : 1:50000, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169