Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min