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Vimentin antibody

KO/KD Orthogonal Overexpression
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in ELISA (ELISA). GTX112661
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in ELISA (ELISA). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661

Cat. No. GTX112661

Reference 23

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629744 / GTX629743, Detection : GTX112661
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , NT2D1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in ELISA (ELISA). GTX112661

GTX112661 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112661

GTX112661 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in ELISA (ELISA). GTX112661

GTX112661 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Annabelle Arlt et al. Orphanet J Rare Dis 2022; 17 (1) : 29 Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.
Application : ICC/IF
Reactivity : Human
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Pan CM et al. Cancer Lett 2020; 493 : 133-142 MicroRNA-7 targets T-Box 2 to inhibit epithelial-mesenchymal transition and invasiveness in glioblastoma multiforme.
Application : WB
Reactivity : Human
Yang P et al. Exp Biol Med (Maywood) 2019; 244 (18) : 1608-1618 ResolvinD1 attenuates high-mobility group box 1-induced epithelial-to-mesenchymal transition in nasopharyngeal carcinoma cells.
Yang P et al. Int J Med Sci 2019; 16 (12) : 1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
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REVIEW

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Application Reference
Annabelle Arlt et al. Orphanet J Rare Dis 2022; 17 (1):29 Novel insights into PORCN mutations, associated phenotypes and pathophysiological aspects.
Application : ICC/IF
Reactivity : Human
Yu-Ting Yen et al. Int J Mol Sci 2021; 23 (1) Down-Regulation of miR-194-5p for Predicting Metastasis in Breast Cancer Cells.
Application : WB
Reactivity : Human
Pan CM et al. Cancer Lett 2020; 493:133-142 MicroRNA-7 targets T-Box 2 to inhibit epithelial-mesenchymal transition and invasiveness in glioblastoma multiforme.
Application : WB
Reactivity : Human
Yang P et al. Exp Biol Med (Maywood) 2019; 244 (18):1608-1618 ResolvinD1 attenuates high-mobility group box 1-induced epithelial-to-mesenchymal transition in nasopharyngeal carcinoma cells.
Yang P et al. Int J Med Sci 2019; 16 (12):1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2):118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.
Application : WB
Reactivity : Human
Ho KH et al. Pharmacol Res 2019; 147:104390 miR-140 targeting CTSB signaling suppresses the mesenchymal transition and enhances temozolomide cytotoxicity in glioblastoma multiforme.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.
Application : WB
Reactivity : Human
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Chen WJ et al. JNB 2017; (Epub) Low folate stress reprograms cancer stem cell-like potentials and bioenergetics metabolism through activation of mTOR signaling pathway to promote in vitro invasion and in vivo tumorigenicity of lung cancers.
Reactivity : Human
Lee WY et al. Int J Mol Sci 2017; 18 (10) Podocalyxin-Like Protein 1 Regulates TAZ Signaling and Stemness Properties in Colon Cancer.
Application : WB
Reactivity : Human
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Hung CY et al. Sci Rep 2017; 7 (1):9166 Nm23-H1-stabilized hnRNPA2/B1 promotes internal ribosomal entry site (IRES)-mediated translation of Sp1 in the lung cancer progression.
Application : WB
Reactivity : Human
Liao C.J. et al. RSC Adv. 2017; 7:29339-29349 Isolation of label-free and viable circulating tumour cells (CTCs) from blood samples of cancer patients through a two-step process: negative selection-type immunomagnetic beads and spheroid cell culture-based cell isolation.
Application : ICC/IF
Reactivity : Human
Fujiki K et al. J Biol Chem 2017; (Epub) Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Application : WB
Reactivity : Human
Tong D et al. Cancer Lett 2016; 389:23-32 * inhibits castration-induced emt in prostate cancer by repressing cox2/pge2/stat3 axis.
Application : WB, IHC-P
Reactivity : Human
Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Zhao B et al. J Proteomics 2015; 112:38-52 Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
Application : WB
Reactivity : Human
Guo J et al. Sci Rep 2015; 5:11660 Investigation of multipotent postnatal stem cells from human maxillary sinus membrane.
Application : ICC/IF
Reactivity : Human
Fock V et al. J Cell Sci 2015; 128 (23):4306-16 Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation.
Application : ICC/IF
Reactivity : Human
Nieuwenhuizen RP et al. PLoS One 2015; 10 (7):e0131756 Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy.
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169