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Vimentin antibody

Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in ELISA (ELISA). GTX112661
Anti-Vimentin antibody used in ELISA (ELISA). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661
Anti-Vimentin antibody used in Western Blot (WB). GTX112661

Cat No. GTX112661

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX629744/GTX629743, Detection: GTX112661

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1 , 293T , HeLa , HepG2 , Neuro2A , NIH-3T3 , Rat2 , C8D30 , C2C12

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952555

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112661

GTX112661 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in ELISA (ELISA). GTX112661

GTX112661 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in ELISA (ELISA). GTX112661

GTX112661 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in IHC (Paraffin sections) (IHC-P). GTX112661

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Vimentin antibody used in Western Blot (WB). GTX112661

GTX112661 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf