GeneTex
United States (US)

Vimentin antibody

KO/KD
GTX112661_40093_20160428_WB_18121410_687.jpg
GTX112661_40093_20160921_IHC-Fr_18121410_668.jpg
GTX112661_40093_20160616_WB_shRNA_watermark_18121410_408.jpg
GTX112661_40093_20170522_IHC-P_M_18121410_561.jpg
GTX112661_40093_20160929_WB_M_18121410_105.jpg
GTX112661_40093_IHC_18121410_220.jpg
GTX112661_40093_WB_M_18121410_137.jpg
GTX112661_40093_20160428_WB_R_18121410_485.jpg
GTX112661_40093_IHC_2_18121410_784.jpg
GTX112661_40093_IFA_18121410_816.jpg
GTX112661_40093_IHC_M_18121410_268.jpg
GTX112661_40093_IHC_3_18121410_858.jpg
GTX112661_40093_20180817_WB_18121410_916.jpg
GTX112661_40093_IHC_4_18121410_420.jpg

Cat No. GTX112661

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

NT2D1 , 293T , HeLa , HepG2 , Neuro2A , NIH-3T3 , Rat2 , C8D30 , C2C12

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ36605 antibody, VIM antibody, vimentin antibody

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX112661_40093_20160428_WB_18121410_687.jpg

GTX112661 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.
The observed M.W. is based on the publication: PMID: 19074872 and 21442631

GTX112661_40093_20160921_IHC-Fr_18121410_668.jpg

GTX112661 IHC-Fr Image

Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112661_40093_20160616_WB_shRNA_watermark_18121410_408.jpg

GTX112661 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

GTX112661_40093_20170522_IHC-P_M_18121410_561.jpg

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112661_40093_20160929_WB_M_18121410_105.jpg

GTX112661 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

GTX112661_40093_IHC_18121410_220.jpg

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112661_40093_WB_M_18121410_137.jpg

GTX112661 WB Image

Vimentin antibody detects VIM protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody (GTX112661) dilution: 1:1000

GTX112661_40093_20160428_WB_R_18121410_485.jpg

GTX112661 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.

GTX112661_40093_IHC_2_18121410_784.jpg

GTX112661 IHC-P Image

Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112661_40093_IFA_18121410_816.jpg

GTX112661 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.

GTX112661_40093_IHC_M_18121410_268.jpg

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at cytosol on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112661_40093_IHC_3_18121410_858.jpg

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112661_40093_20180817_WB_18121410_916.jpg

GTX112661 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112661_40093_IHC_4_18121410_420.jpg

GTX112661 IHC-P Image

Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Vimentin antibody (GTX112661) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169