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Vimentin antibody

Cat. No. GTX20066

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgM

Application

 WB, IHC

Reactivity

 Human
Package
100 μg ($339)
See all Vimentin products

APPLICATION

Application Note

IHC and WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

54 kDa. ( Note )

Product Note

Detects the intermediate filament protein vimentin. The antibody gives a positive reaction with connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human rhabdosarcoma cell line JR1.

Purification

Purified IgM

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 339
Custom Conjugations